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LINE 1 BIOSCIENCES => Diagnosis and gene therapy => Topic started by: PART-1-ES-Antonio on 10 September , 2019, 20:39:16 pm

Title: Diagnosis
Post by: PART-1-ES-Antonio on 10 September , 2019, 20:39:16 pm
Hello everyone. I am Antonio, from Spain.

I was thinking about what to write on the forum, when I just realized that our topic is called "diagnosis and gene therapy". So the idea of writting about diagnosis came to my mind. That is why I started to search and gather information about this topic.

First of all I would like to give a definition about what is the diagnosis, something that I find pretty obvious. According to the wordreference,"the diagnosis is the act of identify a disease".So when I read this I thought, why not to mix gene therapy, that is in what most of us are basing our projects, and the diagnosis. That was when I just found an article about a research done by Kiana Aran, where she explain how her chip is able to identify a mutation in everyone's genome in a matter of minutes using CRISPR Technollogy.

Now I am going to detail how does this technollogy work:
First of all, the chip is based equally on Nano-Technollogy and on Bio-Technollogy. The chip works with Graphene, that is a nano-material composed by carbon atoms, so it is as thin as an atom. Also like this material is quite sensitive, it is able to detect tiny load variations, this open us thousands of doors to work on. And this is in what is based the chip, on recognizing the load variations that take place when the DNA sequence sticks to CRISPR-CAS 9 molecule, which is located directly on the graphene.

In other words, the chip is made up of a thin layer of Graphene, where the CRISPR-CAS 9 molecule is attached, like this molecule has a neutral charge, the graphene does not interact with it. Like most of us know, the CRISPR-CAS 9 molecule is going to search mutations on that DNA chain and when it is detected, the molecule will stick directly on the DNA sequence. This will create a load variation, and this will create that the Graphene detect it and react with it . This process will not take longer than a few minutes.

But most of you, could be asking why this technollogy is that important, if we can do the same with a PCR.
The answer to this question is quitte simple, due to the fact that the PCR takes around 2 hours only to create the copies that in a later process will be use to detect the mutation, so this could long  hours or even days. And this technollogy only needs one simple copy of DNA and wait for about 5 minutes.

https://www.synthego.com/blog/crispr-electronic-biosensor

I hope you are having a great return to rutine.

Antonio Asensio Martin ;Madrid, Spain.