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Ex vivo/In vivo gene therapy

Ex vivo/In vivo gene therapy
« on: 12 August , 2019, 16:13:04 pm »
Hi everyone,

As you probably already read in WORK GUIDE, there are two categories of somatic gene therapy, in vivo and ex vivo.

Short animations to see the difference between ex vivo and in vivo gene therapy:
https://www.youtube.com/watch?v=VnwjJ180FtU (ex vivo)
https://www.youtube.com/watch?v=iUMjzd2T2Dk (in vivo)

Some other links with useful information:
http://www.genetherapynet.com/types-of-gene-therapy.html
https://www.researchgate.net/figure/In-vivo-versus-ex-vivo-gene-therapies-for-the-treatment-of-genetic-diseases-and-cancer_fig1_303531815
http://www.biologydiscussion.com/gene/therapy/gene-therapy-ex-vivo-and-in-vivo-gene-therapy-with-diagram/9969

Now, try to find out why ex vivo is better for the treatment of cancer and in vivo for the treatment of hemophilia. Which one do you think is safer and why?

Share your opinion on this topic with us.

Do not forget, veterans are here for you. :)
Best regards,
Brina from Slovenia

Re: Ex vivo/In vivo gene therapy
« Reply #1 on: 14 August , 2019, 11:45:29 am »
Hello everyone,
I am Antonio,  from Madrid,  Spain. I hope you are having a great holidays.

I would like to give an answer  to Brina's questions.
First of all,  I would like to thank Brina for all the information she has attached to her post.  Due to the fact that it was really useful to answer her questions.

Based on what I have read about this two different types of gene therapy.  I find safer ex vivo gene therapy. I think this method is much safer than the other method because when we are interacting with a cell,  we do not really know how it is going to react because everyone's cells are different.  So if we modify the cells on a laboratory,  it is going to be possible to see how the cell reacts to this modification. But if the interaction is done inside the body, we will not be able to see how it is going to react, even though we have made a recent investigation. 

Trying to give an answer to Brina's first question,  I think that ex vivo is better for the treatment of cancer due to the fact that if you use a viral vector to modify a cancer cell's DNA sequence,  the immune system inside everyone's body(mostly on the blood),  is going to attack that vector, so it will never reach the cell. And if the vector does not reach the cell,  it will not modify the cell's DNA.
But if we see it from the other point of view. Trying to heal a blood disease,  we don't need to care about the immune system because we are going to attack it. Even thought the mutation is not on a Leukocyte, the viral vector is going to be faster than the immune response,  so it will reach the cells and it will do what it is supposed to do. 
So that is why,  based on my opinion and  on what I have read, ex vivo is better for cancer's treatments and in vivo is better for hemophilia's treatments.

Best regards.
Antonio Asensio Martin, Spain.